By Morris R. Cohen, Ernest Nagel
An creation to good judgment and clinical strategy is a faculty point primer on good judgment. This ebook used to be extensively utilized by students specifically these learning economics as an advent to logical suggestion in coaching for highbrow discourse. Ludwig von Mises used to be identified to assert that each one scholars of economics may still learn this ebook as a primary step of their education as economists.
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3. Plastic and glassware used in the assay should be absolutely clean and detergent free. Quartz (silica) spectrophotometer cuvettes should not be used, as the dye binds to this material. Traces of dye bound to glassware or plastic can be removed by rinsing with methanol or detergent solution. 3. 1. Standard Assay Method 1. Pipet between 10 and 100 µg of protein in 100 µL total volume into a test tube. If the approximate sample concentration is unknown, assay a range of dilutions (1, 1:10, 1:100, 1:1000).
Absorbance values increased for each BSA concentration as a second order function of irradiation time. A linear standard curve could be generated from BSA dilutions that were Ultrafast Protein Determinations 25 Fig. 1. Typical standard curves for microwave Lowry and BCA assays. Standard curves were generated with microwave protocols using BSA (Sigma) dissolved in water. BSA samples in 100-µL vol were prepared in triplicate for each assay and were combined with reagent as described in Notes 1 and 2.
Biochem. 208, 44–48. 14. Wilson, C. M. (1979) Studies and critique of amido black 10B, Coomassie Blue R and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis. Analyt. Biochem. 96, 263–278. 15. Zor, T. and Selinger, Z. (1996) Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Analyt. Biochem. 236, 302–308. 16. Redinbaugh, M. G. and Campbell, W. H. (1985) Adaptation of the dye-binding protein assay to microtiter plates.
An Introduction to Logic and Scientific Method by Morris R. Cohen, Ernest Nagel