By Bhanu P. Jena
This can be the 1st booklet to hide the heritage, constitution, and alertness of atomic strength microscopy in telephone biology. awarded within the transparent, well-illustrated type of the Methods in telephone Biology sequence, it introduces the AFM to its readers and allows them to faucet the ability and scope of this know-how to additional their very own study. a realistic laboratory consultant to be used of the atomic strength and photonic strength microscopes, it presents up to date know-how and techniques in strength spectroscopy. it's also a complete and easy-to-follow functional laboratory consultant for using the AFM and PFM in organic examine.
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Additional resources for Atomic Force Microscopy in Cell Biology, Volume 68
6 AFM-generated image of the apical surface of several freshly isolated pancreatic acinar cells demonstrating the presence of microvilli with interspersed pits and depressions. , unpublished). pancreatic acinar cells is amylase. Since all attempts to image amylase being released from pits and depressions in live acinar cells failed, acini were stimulated to secrete in the presence of a gold-tagged amylase antibody. Cells were then immediately ﬁxed using a ﬁxative that retained pit and depression morphology.
Jena and Sang-Joon Cho Departments of Physiology & Pharmacology Wayne State University School of Medicine Detroit, Michigan 48201 I. Introduction II. Methods A. AFM Operation Modes B. AFM Probes C. The Sample III. AFM Studies on Live Cells IV. Identiﬁcation of New Plasma Membrane Structures Involved in Exocytosis A. Apical Plasma Membrane Structures: Pits and Depressions B. Depressions are Fusion Pores C. Transient Fusion Occurs at Depressions V. Future of AFM in the Study of Live Cells References I.
And the surface with a fraction of angstrom precision and, in this way, makes so-called force-clamp measurements possible. By using this technique, unfolding and refolding experiments on small protein structures, such as spectrin, with only four repeats, became possible. , 2000). While approaching this surface with the tip of an AFM cantilever, a single molecule can become attached to the tip, and forces in the range of piconewtons to nanonewtons can be applied to the molecule (Fig. 11). Folded proteins can be stretched to more than 10 times their length, reaching almost their total contour length.
Atomic Force Microscopy in Cell Biology, Volume 68 by Bhanu P. Jena